Sulfolobus Media (download as file)
(ala Zillig)
for 1 liter (revised April 2007)
1. Dissolve in approx. 800 ml distilled water:
a. 3.0 grams Ammonium sulfate: (NH4)2SO4
b. 0.7 grams Glycine
c. 0.5 grams Potassium hydrogen phosphate (potassium phosphate, dibasic): K2HPO4
d. 0.1 grams Potassium chloride: KCl
2. Carbon source:
YS:
a. 1.0 gram Yeast extract
b. 2.0 grams Sucrose
Tryptone (T):
a. 2.0 grams Tryptone
3. Trace elements (solutions are on shelf above the scale):
a. 1 ml of 1M Magnesium chloride / 0.3 M Calcium nitrate solution (pre-made)
b. 200 µl 1% Ferrous sulfate: FeSO4
c. 244 µl 1% Sodium borate: Na2B4O7 • 10 H2O
d. 90 µl Manganese chloride: MnCl2 • 4 H2O
e. 11 µl Zinc sulfate: ZnSO4 • 7 H2O
f. 2.5 µl Cupric sulfate: CuSO4 • 5 H2O
g. 1.5 µl Sodium molybdate: Na2MoO4 • 2 H2O
h. 1.5 µl Vanadyl sulfate: VOSO4 • 5 H2O
i. 0.5 µl Cobalt chloride: CoCl2 • 6 H2O
j. 0.5 µl Nickel sulfate: NiSO4 • 6 H2O
4. Adjust pH to 3.0 – 3.2 with 50% sulfuric acid (approx. 300 µl)
5. Add distilled water to make 1 liter
6. Autoclave on liquid/fluid cycle for 20 minutes
7. May store at room temperature until opened – refrigerate (4° C) after opening