https://www.extremeviruses.org/inhabitable_megan-hanley daily 0.75 2018-09-11 https://images.squarespace-cdn.com/content/v1/5a4af594e9bfdfa8208274d5/1536699896738-337NJSXMPYVQBA8VYUL1/IMG_4563.JPG In/Habitable_Megan Hanley https://images.squarespace-cdn.com/content/v1/5a4af594e9bfdfa8208274d5/1536699896738-337NJSXMPYVQBA8VYUL1/IMG_4563.JPG In/Habitable_Megan Hanley https://images.squarespace-cdn.com/content/v1/5a4af594e9bfdfa8208274d5/1536699956518-WRT8MHH181SYPFOC2FC0/IMG_4568.JPG In/Habitable_Megan Hanley https://images.squarespace-cdn.com/content/v1/5a4af594e9bfdfa8208274d5/1536699957262-R9I0HFM4SWZ01MM62J3G/IMG_4567.JPG In/Habitable_Megan Hanley https://images.squarespace-cdn.com/content/v1/5a4af594e9bfdfa8208274d5/1536699972462-S9371MY1VM60TH1A1CQ6/IMG_4566.JPG In/Habitable_Megan Hanley https://images.squarespace-cdn.com/content/v1/5a4af594e9bfdfa8208274d5/1536699972369-SM7UDNESPYNIP1ZZRWFK/IMG_4565.JPG In/Habitable_Megan Hanley https://images.squarespace-cdn.com/content/v1/5a4af594e9bfdfa8208274d5/1536699979857-CB0ZUF0BSLLOIIG86C73/IMG_4564.JPG In/Habitable_Megan Hanley https://www.extremeviruses.org/sigmaxi_postersession daily 0.75 2018-11-28 https://images.squarespace-cdn.com/content/v1/5a4af594e9bfdfa8208274d5/1543425352336-3F3AXZG61IIKSBZ3MM55/AlyssaSigmaXiPrize2018.JPG SigmaXi PosterSession 16 Nov, 2018 - Alyssa Pratt with her Prize! https://images.squarespace-cdn.com/content/v1/5a4af594e9bfdfa8208274d5/1543425352336-3F3AXZG61IIKSBZ3MM55/AlyssaSigmaXiPrize2018.JPG SigmaXi PosterSession 16 Nov, 2018 - Alyssa Pratt with her Prize! https://images.squarespace-cdn.com/content/v1/5a4af594e9bfdfa8208274d5/1543425581421-1ZY6ODGRVA8R2ZWT09AT/IMG_8708.JPG SigmaXi PosterSession 16 Nov, 2018 https://images.squarespace-cdn.com/content/v1/5a4af594e9bfdfa8208274d5/1543425581148-ZZ5KCROEK0Q1FAXQ02F1/IMG_1168.JPG SigmaXi PosterSession 16 Nov, 2018 https://images.squarespace-cdn.com/content/v1/5a4af594e9bfdfa8208274d5/1543425587537-AEB2I3P3OFSZ6L896R8R/IMG_9369.JPG SigmaXi PosterSession 16 Nov, 2018 https://www.extremeviruses.org/lab-cleaning-7-dec-2018 daily 0.75 2018-12-11 https://images.squarespace-cdn.com/content/v1/5a4af594e9bfdfa8208274d5/1544492541784-BW63F1E760VOBYGU532H/IMG_3336.JPG Lab Cleaning 7 Dec 2018 https://images.squarespace-cdn.com/content/v1/5a4af594e9bfdfa8208274d5/1544492541784-BW63F1E760VOBYGU532H/IMG_3336.JPG Lab Cleaning 7 Dec 2018 https://images.squarespace-cdn.com/content/v1/5a4af594e9bfdfa8208274d5/1544492542417-TGGR1Z6IXC2WRHZD45KE/IMG_8160.JPG Lab Cleaning 7 Dec 2018 https://www.extremeviruses.org/people daily 0.75 2026-03-06 https://images.squarespace-cdn.com/content/v1/5a4af594e9bfdfa8208274d5/1515449304247-Z75ELXKCQW5LA13F9DYA/IMG_8988.jpg People https://images.squarespace-cdn.com/content/v1/5a4af594e9bfdfa8208274d5/528ac610-f01d-4070-ba24-3fcbf729da15/XVL+Group+Lassen+8.JPEG People - Make it stand out The team from the 2023 trip to Lassen Volcanic National Park https://images.squarespace-cdn.com/content/v1/5a4af594e9bfdfa8208274d5/1566533071115-Z3679MBCIQPQD2TE43I2/IMG_5649.jpg People The team from the 2018 trip to Lassen Volcanic National Park https://images.squarespace-cdn.com/content/v1/5a4af594e9bfdfa8208274d5/1518565058704-JJSXXYFA1UI3UDNJ8TJ6/IMG_20180126_132912_1.jpg People Ken Stedman Ph.D. Principal Investigator B.S. Chemical Engineering, Stanford, 1987 Ph.D. Molecular & Cell Biology, UC Berkeley, 1996 Virus hunter, science communicator, and money grubber. Being a Dad, biker, and trying not to grow up. Curriculum Vitae https://images.squarespace-cdn.com/content/v1/5a4af594e9bfdfa8208274d5/1518564848216-9TTGQAOUVR3BZ0EL1POA/MVIMG_20180213_153246.jpg People Ignacio "Nacho" de la Higuera, Ph.D. Research Associate B.S. Biology, University of Granada, Spain, 2008 Ph.D Molecular Biology, Autonomous University of Madrid, Spain, 2014 Virus Origins and Evolution. Cruciviruses. Fuselloviruses. Tato-pani enthusiat. Onsen-connoisseur. Playing drums and weirdo music. https://images.squarespace-cdn.com/content/v1/5a4af594e9bfdfa8208274d5/2757d12f-6f8a-4346-a74c-e70cbde33ec0/IMG_5748.jpg People - Make it stand out Jono Abshier B. S. Biomedical Science, University of Texas Rio Grande Valley, 2019 Doctoral Candidate, Portland State University - Biology SSVs and Saccharolobus. Leisure reading, mushroom hunting, cooking and dancing when no one is watching in lab. https://images.squarespace-cdn.com/content/v1/5a4af594e9bfdfa8208274d5/1dc61cc4-e066-49c6-b1e0-50afcb3deecb/IMG_7436+Medium.jpg People - Make it stand out David Weiyi Noeckel B. A. Biochemistry and Molecular Biology, Reed College, 2020 Doctoral Candidate, Portland State University - Biology Crucivirus Replication protein and DNA biochemistry. Cat-wrangling, sketching, cooking dim sum, and pulling noodles. https://images.squarespace-cdn.com/content/v1/5a4af594e9bfdfa8208274d5/28d78ce3-cda9-471c-a296-ce1fc2a049d2/IMG_0522.jpg People https://images.squarespace-cdn.com/content/v1/5a4af594e9bfdfa8208274d5/1529868583288-YEO4SZ6FTRZP9A5JU9K7/m-0183e.jpg People Brenda Watt Ph.D. University of Pennsylvania Previously Senior Scientist, Stonestable, Inc., now Refeyn Ltd. Worked to develop a marketable vaccine preservation method using silica for Dr. Stedman's startup company StoneStable, Inc. https://images.squarespace-cdn.com/content/v1/5a4af594e9bfdfa8208274d5/1530314729859-8FCL1GB9QU9YT5ZGPB5V/3739521961_55431c1029_z.jpg People John Howieson M.D. U. Kansas Dr. Howieson, a retired physician, has never stopped learning, and continues to attend classes at Portland State. He is also a good friend of the lab and extremely generous patron of our research and the Biology department. https://images.squarespace-cdn.com/content/v1/5a4af594e9bfdfa8208274d5/1530314341686-41DOEPFBWDFJSKR5LXV9/david2.jpg People David Schwartz The Chief Cryptographer at Ripple, a popular cryptocurrency, Dave is a friend and supporter of the Stedman Lab. https://images.squarespace-cdn.com/content/v1/5a4af594e9bfdfa8208274d5/1561229746607-K5RTBCJALFRTPRYQ2ES9/ssv1_Cryo.jpg People - Make it stand out Allison Stenger Ph.D. https://images.squarespace-cdn.com/content/v1/5a4af594e9bfdfa8208274d5/1518564433269-J1YH9SIITBQFYYNVEFZ8/00100dPORTRAIT_00100_BURST20180213151223826_COVER.jpg People George Kasun B.S. Biology, 20??, UC Davis Ph.D. 2021 BSL-RDV Rep protein. Fishing a lot. https://images.squarespace-cdn.com/content/v1/5a4af594e9bfdfa8208274d5/1518563741259-W3LCXSC17N4PD9FD7JXP/David+Goodman People David Goodman B.S. Biology, Portland State, 2014 M.S. Biology, Portland State, 2018 Genomic characterization and most things pertaining to SSV1 & SSV10. Don't talk to me I'm singing. Now Laboratory Preparator, Portland State University. https://images.squarespace-cdn.com/content/v1/5a4af594e9bfdfa8208274d5/1530306033548-JPY0V5158JNSJK4XJNQ7/00100dPORTRAIT_00100_BURST20180629134645463_COVER.jpg People Nichole Procter B.S. Biology, Portland State, 2020 SSV mutants. Gardening and being a mom to three chihuahuas. Ph.D. Program at U.C. Irvine. https://images.squarespace-cdn.com/content/v1/5a4af594e9bfdfa8208274d5/1518564235552-6FXHDRXF6UJMLPOAN9KE/00100srPORTRAIT_00100_BURST20180119141433150_COVER.jpg People Ellis Torrance B.S. Biology, Portland State, 2018 2018 McNair Scholar In the Stedman lab worked on Crucivirus host elucidation. Now a DOE Computational Science Fellow in Ph.D. program at UNC Greensboro. https://images.squarespace-cdn.com/content/v1/5a4af594e9bfdfa8208274d5/1518564194377-WV9QG0YQHY1N9ZN9H5Q7/Sara+Protzek People Sara Protzek B.S. Biology, Portland State, 2017 In the Stedman Lab, Sara worked with Cruciviruses. She now works as a lab technician at Oregon Health and Science University. https://images.squarespace-cdn.com/content/v1/5a4af594e9bfdfa8208274d5/1521833962830-DH0M1ZJVTGKA0YCEZHGL/00100dPORTRAIT_00100_BURST20180323115004025_COVER.jpg People Max Larson B.S. Biology, Portland State, 2016 BSL-RDHV Rep protein expression. Hiking and hanging out with my cat. Clinical Medical Technology https://images.squarespace-cdn.com/content/v1/5a4af594e9bfdfa8208274d5/1521774383572-T4JFA84F5KB1AG6ZFIPA/29249355_1836939859704164_4763844756830683136_o.jpg People Rita Clare B.S. Biology, Portland State, 2018 B.S. Industrial Design, University of Oregon, 2014 SSV capsid expression, virus purification, Cas4 cloning and expression. Now working at the National Renewable Energy Research Laboratory in Golden, Colorado. NSF GRFP Recipient 2020. Ph.D. program at Montana State University https://images.squarespace-cdn.com/content/v1/5a4af594e9bfdfa8208274d5/1521773814076-AT6BX389G5YU75N89ZOS/00100dPORTRAIT_00100_BURST20180223132225602_COVER.jpg People Thymara van Kuringen B.S. Applied Science, 2018 Fontys University of Applied Sciences, Netherlands International Undergraduate Student SSV1 mutant quantification using qPCR. Biking and swimming (essential for a Dutch person) https://images.squarespace-cdn.com/content/v1/5a4af594e9bfdfa8208274d5/1518564509433-ULHW4896KJNW8TZ4KEWR/image-asset.jpeg People Lorenzo Carré Former International Masters Student from France who worked on SSV infectivity quantification. https://images.squarespace-cdn.com/content/v1/5a4af594e9bfdfa8208274d5/1561229936959-1QBLAIMPTGPENH2VPCVC/ssv1_Cryo.jpg People Suzie Wallace B.S. Biology, Portland State University https://images.squarespace-cdn.com/content/v1/5a4af594e9bfdfa8208274d5/1518564166301-NMY9MKYWFQU0HGILCFU3/00100dPORTRAIT_00100_BURST20180119162002800_COVER.jpg People Courtney Micheletti B.S. Biology, Portland State, 2018 Parasite egg preservation ("poopy worms"). Plants, hiking, fishing and generally being a wilderness woman. Research Technician at OHSU https://images.squarespace-cdn.com/content/v1/5a4af594e9bfdfa8208274d5/1518731588581-7HX5FECN1T8QD2AMVA16/00100dPORTRAIT_00100_BURST20180215134904257_COVER.jpg People Amber Maluenda B.S. Biology, Portland State, 2020 BUILD EXITO Scholar Crucivirus capsid protein expression Hanging out with my daughter and crocheting. https://images.squarespace-cdn.com/content/v1/5a4af594e9bfdfa8208274d5/1566533275306-0F0BUP06EXOH39PRXB1Q/CorannaAction2_Jul19.jpeg People Coranna Akdemirby ASE High School Student https://images.squarespace-cdn.com/content/v1/5a4af594e9bfdfa8208274d5/1518635013122-S8JAW42P7F82QLRBLP5D/00100dPORTRAIT_00100_BURST20180213160634820_COVER.jpg People Lena Nguyen B.S. Biology, Portland State, 2017 SSV mutant infectivity. Pokémon hunting and hiking. https://images.squarespace-cdn.com/content/v1/5a4af594e9bfdfa8208274d5/1530305744454-7C8T3DE4DB24OGTTBGHW/IMG_20180629_134853.jpg People James Musser B.S. Biology, Portland State, 2019 Sulfurisphaera stuff Hiking with my dog and that's it. https://images.squarespace-cdn.com/content/v1/5a4af594e9bfdfa8208274d5/1518634914230-MH1UIE2TMRE0AUDQSF7K/00100dPORTRAIT_00100_BURST20180214104803231_COVER.jpg People Diana Demchenko B.S. Biology, Portland State, 2019 2017 Built Exito Scholar , 2018 Lester Newman Scholar Long-term storage of virus particles, antiviral properties of nanoparticles, Saccharolobus bioinformatics. Chicken hugging enthusiast. https://images.squarespace-cdn.com/content/v1/5a4af594e9bfdfa8208274d5/1530296746126-FEWJEPJT6X48KWQ705EZ/00100dPORTRAIT_00100_BURST20180628154317001_COVER.jpg People Camden Hoover B.S. Newman University, 2019 2018 REU Student Worked on parasitic worm egg preservation and imaging. Soon to start graduate school. https://images.squarespace-cdn.com/content/v1/5a4af594e9bfdfa8208274d5/1529866083266-K4OW0NMHJGW1ITRAZPWB/00100dPORTRAIT_00100_BURST20180622140137638_COVER.jpg People Swati Pandruvada A former REU student from Case Western, Swati likes to spend her summers with us studying parasitic worm egg preservation. Swati started medical school in the Fall of 2019. https://images.squarespace-cdn.com/content/v1/5a4af594e9bfdfa8208274d5/1526588859321-BKPNGJXSKXEJSQV93HLJ/MVIMG_20180517_132231+%281%29.jpg People Ahn Le-Cook B.S. Biology, Portland State, 2016 Studying a new Sulfurisphaera species Watching sci-fi and cooking. Now working at the VA Medical Center in Portland as a lab technician. https://www.extremeviruses.org/sarscov2-blog daily 0.75 2022-03-13 https://images.squarespace-cdn.com/content/v1/5a4af594e9bfdfa8208274d5/1584048666792-7XCKCVEJ286U4TD5PL6V/coronavirus.jpg SARS-CoV-2 Blog https://www.extremeviruses.org/sarscov2-blog/2022/3/12/my-thoughts-on-masking monthly 0.5 2022-03-13 https://www.extremeviruses.org/sarscov2-blog/2021/8/30/what-are-my-thoughts-on-booster-shots monthly 0.5 2021-08-30 https://www.extremeviruses.org/sarscov2-blog/2021/8/14/thoughts-on-schools-vaccines-and-the-delta-variant monthly 0.5 2021-08-14 https://www.extremeviruses.org/sarscov2-blog/2021/6/6/why-covid-19-mrna-vaccines-would-not-be-reverse-transcribed-into-dna monthly 0.5 2021-06-06 https://www.extremeviruses.org/sarscov2-blog/2021/5/31/will-the-covid-19-sars-cov-2-vaccines-change-your-genetic-material monthly 0.5 2021-06-01 https://www.extremeviruses.org/sarscov2-blog/2021/4/30/covid-vaccine-faq-mini-videos monthly 0.5 2021-05-01 https://www.extremeviruses.org/sarscov2-blog/2021/4/14/thoughts-on-fend-hellofenddotcom-for-covid-19 monthly 0.5 2021-04-14 https://www.extremeviruses.org/sarscov2-blog/2020/3/28/thoughts-on-wonder-drug-combination-hydroxychloroquine-and-azithromycin monthly 0.5 2020-03-29 https://www.extremeviruses.org/sarscov2-blog/2020/3/23/transmission-and-airplanes monthly 0.5 2020-03-23 https://www.extremeviruses.org/sarscov2-blog/2020/3/22/food-safety-in-the-time-of-covid-19-take-outdelivery monthly 0.5 2020-03-23 https://www.extremeviruses.org/sarscov2-blog/2020/3/15/question-on-coronaviruses-in-local-bats monthly 0.5 2020-03-16 https://www.extremeviruses.org/sarscov2-blog/2020/3/14/sars-cov-2-on-surfaces-medrxiv-preprint monthly 0.5 2020-03-18 https://www.extremeviruses.org/sarscov2-blog/2020/3/12/comments-on-what-i-am-doing-for-the-upcoming-covid-19-coronavirus-pandemic monthly 0.5 2020-03-15 https://www.extremeviruses.org/sarscov2-blog/2020/3/12/critical-reading-of-covid-2-advice-letter monthly 0.5 2020-03-12 https://images.squarespace-cdn.com/content/v1/5a4af594e9bfdfa8208274d5/1584047847128-41NL3SAVS9J96BWT6ASM/COVID-19+vs+Cold+vs+Flu.jpg SARS-CoV-2 Blog - Critical reading of COVID-2 "Advice" letter https://www.extremeviruses.org/sarscov2-blog/tag/COVID-19 monthly 0.5 https://www.extremeviruses.org/sarscov2-blog/tag/SARS-CoV-2 monthly 0.5 https://www.extremeviruses.org/sarscov2-blog/tag/Coronavirus monthly 0.5 https://www.extremeviruses.org/sarscov2-blog/tag/coronavirus monthly 0.5 https://www.extremeviruses.org/sarscov2-blog/tag/mRNA monthly 0.5 https://www.extremeviruses.org/sarscov2-blog/tag/Vaccines monthly 0.5 https://www.extremeviruses.org/sarscov2-blog/tag/reverse+transcription monthly 0.5 https://www.extremeviruses.org/tem-screening-for-viruslike-particles daily 0.75 2018-01-06 https://www.extremeviruses.org/publications daily 0.75 2024-09-16 https://images.squarespace-cdn.com/content/v1/5a4af594e9bfdfa8208274d5/1522796078164-HVN3TZFN665NF7AEJ0O9/IMG_8997.jpg PUBLICATIONS https://www.extremeviruses.org/sulfolobus-media daily 0.75 2018-01-06 https://www.extremeviruses.org/sulfolobus-transformiation-via-electroporation daily 0.75 2018-01-06 https://www.extremeviruses.org/sulfolobus-freezer-stock-prep daily 0.75 2018-01-06 https://www.extremeviruses.org/in-the-press daily 0.75 2018-07-07 https://images.squarespace-cdn.com/content/v1/5a4af594e9bfdfa8208274d5/1530315322266-9RUIZDEKAE5AQ69YRBT9/Killer+space+viruses%21.jpg In the Press https://www.extremeviruses.org/genomic-dna-prep-from-sulfolobus daily 0.75 2018-01-06 https://www.extremeviruses.org/home daily 1.0 2024-09-16 https://images.squarespace-cdn.com/content/v1/5a4af594e9bfdfa8208274d5/1523816965481-X3P0K66Z4AB76O43UJ3Q/IMG_9078+copy.jpg Home https://images.squarespace-cdn.com/content/v1/5a4af594e9bfdfa8208274d5/1521757988367-G3GAFJ0605K3HU6NG2C9/C7jRw2FVQAAtq_R.jpg Home "Stanley", our 3D printed model of an SSV1, in front of Chinoike Jigoku. Translating to Blood Pond Hell, this geothermal hot spring in Beppu, Japan is where the first SSV was discovered. It is only one of the geothermal hot springs around the world where many more SSVs and similar viruses have been found. https://images.squarespace-cdn.com/content/v1/5a4af594e9bfdfa8208274d5/1522522771944-6CBYEI5KU9JN1L62DEE5/crucifixion.001.jpeg Home Genome and hypothetical structure of a crucivirus https://images.squarespace-cdn.com/content/v1/5a4af594e9bfdfa8208274d5/1529778590097-OTOJ3WNIX7BWC1Y058KV/poopyegg.jpg Home The silified egg of Trichuris suis under a scanning electron microscope (SEM). https://www.extremeviruses.org/sulfolobus-plates daily 0.75 2018-01-06 https://www.extremeviruses.org/pouring-a-sulfolobus-lawn-for-halo-assays daily 0.75 2018-01-06 https://www.extremeviruses.org/poopy-worms daily 0.75 2019-06-23 https://images.squarespace-cdn.com/content/v1/5a4af594e9bfdfa8208274d5/1529869766348-M0ZQODK6X4KHIT88OCP0/poopyegg2.jpg Parasitic Worm Egg Preservation https://images.squarespace-cdn.com/content/v1/5a4af594e9bfdfa8208274d5/1521954223225-NVQM70TY6UF94F1ESG4Z/wormdiscover.jpg Parasitic Worm Egg Preservation A mature Trichuris worm https://images.squarespace-cdn.com/content/v1/5a4af594e9bfdfa8208274d5/1521954302916-NEXJBH40G403DTVWVTJG/trichuris40x.jpg Parasitic Worm Egg Preservation A Trichuris egg https://images.squarespace-cdn.com/content/v1/5a4af594e9bfdfa8208274d5/1521951505740-3U27NIO6ZUHGDNNC92L0/trichuris_lifecycle.gif Parasitic Worm Egg Preservation The life cycle of Trichuris trichiura involves stages both inside and outside of the body. https://images.squarespace-cdn.com/content/v1/5a4af594e9bfdfa8208274d5/1529869185755-P2YU3JSWLL6RCY5RAOYQ/poopyegg.jpg Parasitic Worm Egg Preservation An SEM image of a silicified T. suis egg at 9000x magnification https://images.squarespace-cdn.com/content/v1/5a4af594e9bfdfa8208274d5/1529869062468-P9GUTBDUFUGQKNTXIRLA/image-asset.jpeg Parasitic Worm Egg Preservation Some of the T. suis eggs that we work with under a light microscope. https://www.extremeviruses.org/lab-protocol daily 0.75 2020-10-12 https://images.squarespace-cdn.com/content/v1/5a4af594e9bfdfa8208274d5/1515304790148-HMSXE858HSRJHJPM5OJZ/CSBM.PNG Lab Protocols https://www.extremeviruses.org/contact daily 0.75 2018-04-03 https://images.squarespace-cdn.com/content/v1/5a4af594e9bfdfa8208274d5/1514868975773-N7RDJW4NUJIDIQCJT4Y2/DK3_Jul17.JPG Contact https://images.squarespace-cdn.com/content/v1/5a4af594e9bfdfa8208274d5/1514873899828-HFRIUUSYH2HRRVQ9WF49/srtclabmap.jpg Contact - Stedman Lab Location Portland State University Center for Life in Extreme Environments Department of Biology Room 466, SRTC https://www.extremeviruses.org/plasmidvirus-prep-from-sulfolobus daily 0.75 2018-01-06 https://www.extremeviruses.org/new-page-1 daily 0.75 2019-06-23 https://www.extremeviruses.org/virus-and-vaccine-preservation daily 0.75 2019-06-23 https://images.squarespace-cdn.com/content/v1/5a4af594e9bfdfa8208274d5/1530297910873-7DQWFM3TM6RFWEA1XG6Y/Screen+Shot+2018-06-14+at+4.48.04+PM.png Virus and Vaccine Preservation https://images.squarespace-cdn.com/content/v1/5a4af594e9bfdfa8208274d5/1530144755859-GXYC4HQZGYJ6W7ABJJFK/Screen+Shot+2018-06-27+at+5.04.31+PM.png Virus and Vaccine Preservation James Laidler, M.D., Ph.D, sampling from a hot spring in Lassen Volcanic National Park https://images.squarespace-cdn.com/content/v1/5a4af594e9bfdfa8208274d5/1530144595270-6VZM55QYXDBUK8WDODXS/Screen+Shot+2018-06-27+at+5.05.26+PM.png Virus and Vaccine Preservation A comparison between T4 Bacteriophage that hasn't been coated in silica (Top), and the somewhat non-distinct blobs formed by the silicification of the same phages (Bottom). Not exactly the level of distinction you want to see if you're hoping to distinguish virus particles from rock particles. https://images.squarespace-cdn.com/content/v1/5a4af594e9bfdfa8208274d5/1529872261060-IIF6SNW9V1VNNU5Z3SYW/cold-chain.jpg Virus and Vaccine Preservation A healthcare worker in South Sudan carrying the typical kind of rural vaccine transport - an insulated cooler. Credit: UNICEF https://images.squarespace-cdn.com/content/v1/5a4af594e9bfdfa8208274d5/1530204013767-H48JMIJ47NDHLKWEWOWA/Screen+Shot+2018-06-28+at+9.39.45+AM.png Virus and Vaccine Preservation Plaque assays done using Yellow Fever Virus after 3 days in silica treatment (bottom row) and the control without silica (top row). https://www.extremeviruses.org/ase-symposium-17-aug-2018 daily 0.75 2018-08-18 https://images.squarespace-cdn.com/content/v1/5a4af594e9bfdfa8208274d5/1534543524239-RS47ZBT0WAVMRX4E2SKX/AlyssaPoster_ASE18.JPG ASE Symposium, 17 Aug 2018 Alyssa's Poster https://images.squarespace-cdn.com/content/v1/5a4af594e9bfdfa8208274d5/1534543251994-EAMFH7Q96RPJR52VN4B1/IMG_4495.JPG ASE Symposium, 17 Aug 2018 Alyssa's Awesome Talk (with bystander) https://images.squarespace-cdn.com/content/v1/5a4af594e9bfdfa8208274d5/1534543365820-4HVNY33HNY9IELL4J72Z/AlyssaMentors_ASE18.JPG ASE Symposium, 17 Aug 2018 Alyssa and her mentors. https://images.squarespace-cdn.com/content/v1/5a4af594e9bfdfa8208274d5/1534543464470-RTXHPTDJZ4QD6Z7ZF74R/CruciCrew_ASE18.JPG ASE Symposium, 17 Aug 2018 The CruciCrew, after Alyssa's talk. https://www.extremeviruses.org/microtalk-podcast daily 0.75 2018-08-30 https://www.extremeviruses.org/nanoparticle-antivirals daily 0.75 2018-07-25 https://images.squarespace-cdn.com/content/v1/5a4af594e9bfdfa8208274d5/1530296480390-RKZ7AHU9EHDEAMBPWFI3/ken+and+marilyn.jpg Nanoparticle Antivirals Dramatic reenactment of that fateful beer that Dr. Stedman and Dr. Mackiewicz had https://images.squarespace-cdn.com/content/v1/5a4af594e9bfdfa8208274d5/1530310458913-XGRSKW6PI5I27S14LR6D/12951_2005_Article_24_Fig3_HTML.jpg Nanoparticle Antivirals Images from a 2005 paper by Jacaman et al., showing the binding of AgNPs to glycoproteins on the surface of HIV-1 https://images.squarespace-cdn.com/content/v1/5a4af594e9bfdfa8208274d5/1530306903097-L7EAE1I8ZVLZWXVM9XBQ/Screen+Shot+2018-06-29+at+2.12.09+PM.png Nanoparticle Antivirals Plaque assays using Saccharolobus solfataricus infected with WT SSV1. The plate on the left, incubated without AgNPs, shows a significantly higher number of plaques than the plate incubated with the AgNPs (plate on the right). These were the promising results that showed AgNps could be used as an antiviral. https://www.extremeviruses.org/lunch-with-friend-of-the-stedman-lab daily 0.75 2018-08-23 https://images.squarespace-cdn.com/content/v1/5a4af594e9bfdfa8208274d5/1535058383049-D82ELXTTGGULJIO8UCY1/20180823_LunchWithAlison.jpg Lunch with friend of the Stedman Lab https://www.extremeviruses.org/grant daily 0.75 2018-08-25 https://images.squarespace-cdn.com/content/v1/5a4af594e9bfdfa8208274d5/1535158291069-GJTOQ7KP9YIGSYGD5HO2/Messer.png Grant! Bill Messer, OHSU, new Collaborator! https://www.extremeviruses.org/cruciviruses daily 0.75 2024-09-26 https://images.squarespace-cdn.com/content/v1/5a4af594e9bfdfa8208274d5/1515529202461-T95V0FP5NV9PLUKIR3ID/IMG_9058.JPG Cruciviruses https://images.squarespace-cdn.com/content/v1/5a4af594e9bfdfa8208274d5/1521931477830-R532837SR6L7HY48M64G/IMG_8846.jpg Cruciviruses https://images.squarespace-cdn.com/content/v1/5a4af594e9bfdfa8208274d5/1521930521619-ON9G1PEM2QRBR98NCD2M/image-asset.jpeg Cruciviruses Cruciviruses may be the result of a recombination between a ssDNA virus and a ssRNA virus. The capsid-encoding genes of Cruciviruses are homologous to RNA viruses, while the Rep encoding genes are homologous to ssDNA viruses. Cruciviridae was a proposed taxonomic name for cruciviruses, but they remain unclassifiable! https://images.squarespace-cdn.com/content/v1/5a4af594e9bfdfa8208274d5/1516502142490-LA6IZ9MRZAEJJNFS9KO5/BSLsample+%281%29.gif Cruciviruses Scooping up environmental samples from Boiling Springs Lake in Lassen Volcanic National Park. Due to unstable hot spots in the earth around the lake, sampling is done from afar, and requires some creativity. This is where BSL-RDHV was discovered, and where we hope to find more viral sequences like it. https://images.squarespace-cdn.com/content/v1/5a4af594e9bfdfa8208274d5/1516502566833-RX40672TOKRW14YF7MUD/crucicrew.jpg Cruciviruses Part of the "crucicrew" on our 2017 trip to Lassen. https://images.squarespace-cdn.com/content/v1/5a4af594e9bfdfa8208274d5/1526247043344-L8IGTXE8U0GBKCAIEEPY/Screen+Shot+2018-05-13+at+2.04.25+PM.png Cruciviruses Mill creek in Woodburn (OR) is where some cruciviruses were found. The crucicrew takes frequent trips here for sampling in hopes of finding more crucis and their hosts https://images.squarespace-cdn.com/content/v1/5a4af594e9bfdfa8208274d5/1526247009112-RTLZWUPK2L2SGVKPVSKQ/IMG_9096.JPG Cruciviruses Dr. Nacho and Ellis Torrance testing the pH of soil samples from Drakesbad Marsh, Lassen Volcanic Natl. Park, Northern California. https://www.extremeviruses.org/sulfolobus daily 0.75 2024-09-26 https://images.squarespace-cdn.com/content/v1/5a4af594e9bfdfa8208274d5/1516505618727-K8JM1P2MMITMJ3V8X85I/IMG_9223+%282%29.jpg Sulfolobus / Saccharolobus https://images.squarespace-cdn.com/content/v1/5a4af594e9bfdfa8208274d5/1515794568348-BCBP8XZ43BGTA7PZKUL4/KMSRabbitCreek.jpg Sulfolobus / Saccharolobus Dr. Stedman sampling Sulfolobus in the Rabbit Creek Hot Spring Area in Yellowstone National Park, United States, 2001 https://images.squarespace-cdn.com/content/v1/5a4af594e9bfdfa8208274d5/1528064691654-Z1VDK2DOLNWE9XLPKFWF/kuroyu_6609.jpg Sulfolobus / Saccharolobus Eggs turned black from boiling in the Kuroyu Hot Spring (source) https://images.squarespace-cdn.com/content/v1/5a4af594e9bfdfa8208274d5/1528064678997-ORHLIAQ67MB7H05W4OGP/caption.jpg Sulfolobus / Saccharolobus Kuroyu Hot Springs, where our sample came from. https://images.squarespace-cdn.com/content/v1/5a4af594e9bfdfa8208274d5/1528064398165-77N4LD1L39RR2HNOXH0C/Screen+Shot+2018-06-03+at+3.19.24+PM.png Sulfolobus / Saccharolobus A YS media plate growing the unknown species (upper left), next to other known Sulfolobales. Note the "fuzzy" undefined edges. https://images.squarespace-cdn.com/content/v1/5a4af594e9bfdfa8208274d5/1528065365679-XSH8F25B78UMWL2M2OF3/Screen+Shot+2018-06-03+at+3.30.27+PM.png Sulfolobus / Saccharolobus The first and best image of the hyperflagellated S. mobilis https://www.extremeviruses.org/new-page-2 daily 0.75 2018-08-16 https://images.squarespace-cdn.com/content/v1/5a4af594e9bfdfa8208274d5/1534204194117-49KMTUW6KHLZSM7OSIL0/Woodburn_Sample.JPG Woodburn, OR 13 Aug 2018 Ellis Torrance Collecting Samples in Woodburn, OR https://images.squarespace-cdn.com/content/v1/5a4af594e9bfdfa8208274d5/1534204219052-18KQWF66SQ3DDDUP9FCZ/LargeScaleVirology.JPG Woodburn, OR 13 Aug 2018 Collecting 12,000 year old soil https://images.squarespace-cdn.com/content/v1/5a4af594e9bfdfa8208274d5/1534204269942-B28WY9S0CGMDT9535NW7/DrAmieDrNacho_Woodburn.jpg Woodburn, OR 13 Aug 2018 "Dr. Nacho" and "Dr. Amie" finding old bones. https://images.squarespace-cdn.com/content/v1/5a4af594e9bfdfa8208274d5/1534204135701-KNK9JBLUKQC9J1R0YM3L/WoodburnPaleontology.JPG Woodburn, OR 13 Aug 2018 Paleontology Sign in Woodburn, OR https://images.squarespace-cdn.com/content/v1/5a4af594e9bfdfa8208274d5/1534204244988-4SML7W8NPW07EXZ5MCEV/EllisWoodburn.jpg Woodburn, OR 13 Aug 2018 Ellis Torrance finding old bones https://images.squarespace-cdn.com/content/v1/5a4af594e9bfdfa8208274d5/1534461399784-EGJU552PI1XOF4KBPHVB/20180813_CruciCrewWoodburn.jpg Woodburn, OR 13 Aug 2018 Crucicrew and helpers, Mill Creek Woodburn https://images.squarespace-cdn.com/content/v1/5a4af594e9bfdfa8208274d5/1534434231632-CJCPESPHIKWZI3DK6X88/Ken_+Kaleiha_WoodburnAug18.jpg Woodburn, OR 13 Aug 2018 Ken with budding virologist (and Stanley the SSV). https://www.extremeviruses.org/diana-featured-on-psu-foundation-website daily 0.75 2019-08-14 https://images.squarespace-cdn.com/content/v1/5a4af594e9bfdfa8208274d5/1565806994029-K8Z0G3RN0REUJMJ4XFHP/psuf_web_diana_hero.jpg Diana Featured on PSU Foundation Website https://www.extremeviruses.org/ellis-leaving-party daily 0.75 2018-08-29 https://images.squarespace-cdn.com/content/v1/5a4af594e9bfdfa8208274d5/1535564554238-GVMI4WGWXLO2868IV210/20180828_174027_HDR.jpg Ellis' Leaving Party Meeting at "Science Building 3" https://images.squarespace-cdn.com/content/v1/5a4af594e9bfdfa8208274d5/1535565577858-L97PL9UXGSNG762QHDWA/image1+%281%29.jpeg Ellis' Leaving Party Ellis with her unique Geminivirus lamp! May it light your way to continuing brilliance! https://www.extremeviruses.org/research daily 0.75 2024-09-26 https://images.squarespace-cdn.com/content/v1/5a4af594e9bfdfa8208274d5/1514868470326-F889W3O193HXI4VZX6M6/BSL_MudPot2.JPG Sulfolobus Spindle-shaped Viruses https://images.squarespace-cdn.com/content/v1/5a4af594e9bfdfa8208274d5/1515527074797-TBLDZIFT27XF0SN0H10S/SSV1.jpg Sulfolobus Spindle-shaped Viruses SSV1 Structure from Stedman et al. 2015 https://images.squarespace-cdn.com/content/v1/5a4af594e9bfdfa8208274d5/1521945146005-749CVR85HCWOZZS6VPAG/3745986298879601128.jpg Sulfolobus Spindle-shaped Viruses A halo assay testing the infectivity of SSV10 mutants. Notice the difference in border sharpness, and diameter each of the mutants have. https://images.squarespace-cdn.com/content/v1/5a4af594e9bfdfa8208274d5/1515527878149-MQ5SCJVE213AZQURCS13/ssv1+genome.jpg Sulfolobus Spindle-shaped Viruses The SSV1 genome showing A) conservation across 11 Fuselloviridae, and B) the infectivity of host Saccharolobus solfataricus by different SSV1 mutants. From Iverson et al., 2017 https://images.squarespace-cdn.com/content/v1/5a4af594e9bfdfa8208274d5/1524949200676-DFBR5VL1QTG78CZC785M/Screen+Shot+2018-04-28+at+1.26.40+PM.png Sulfolobus Spindle-shaped Viruses TEM & rendering of SSV1 budding through the host cell membrane and S-layer. From the 2016 Quemin et al. paper Eukaryotic-Like Virus Budding in Archaea. These beautiful images show how SSV1 fuses with the host cell membrane during egress. https://images.squarespace-cdn.com/content/v1/5a4af594e9bfdfa8208274d5/1529791697858-7X5SA6YJSSXWF4J1F1XU/Screen+Shot+2018-06-23+at+3.05.47+PM.png Sulfolobus Spindle-shaped Viruses The strangely "cigarillo" shaped viruses that result from the deletion of the vp3 capsid gene (left), as compared to the wild type SSV1 (right). https://images.squarespace-cdn.com/content/v1/5a4af594e9bfdfa8208274d5/1530227825583-1WX7DC4U0ODERHEBZYGG/Screen+Shot+2018-06-28+at+4.15.09+PM.png Sulfolobus Spindle-shaped Viruses A figure from our 2017 paper Genetic Analysis of the Major Capsid Protein of the Archaeal Fusellovirus SSV1: Mutational Flexibility and Conformational Change, showing the residue conservation of the vp1 N-terminus across different SSVs. https://images.squarespace-cdn.com/content/v1/5a4af594e9bfdfa8208274d5/1530999454680-ZQC5FS94BMKMOU9CGT2S/Screen+Shot+2018-07-07+at+2.10.54+PM.png Sulfolobus Spindle-shaped Viruses A toroidal multimeric Cas4 protein from Sulfolobus solfataricus, determined by Yakunin et al. in 2013. A monomer of this protein has a similar structure to an uncharacterized ORF in SSV10 that we are investigating. https://images.squarespace-cdn.com/content/v1/5a4af594e9bfdfa8208274d5/1523823812804-68SGMAZM9JM731EZN1J3/image-asset.jpeg Sulfolobus Spindle-shaped Viruses https://images.squarespace-cdn.com/content/v1/5a4af594e9bfdfa8208274d5/1523823794623-QSIRGM0T2IFF3Y4KLR92/2155.jpg Sulfolobus Spindle-shaped Viruses https://www.extremeviruses.org/new-page-3 daily 0.75 2019-09-12 https://images.squarespace-cdn.com/content/v1/5a4af594e9bfdfa8208274d5/1568325574552-KWO36GQOE5B3WRS3GBBH/NachoASV19.JPG ASV 2019, Nacho's Talk Nacho’s talk at ASV, Minneapolis, Minnesota, 20 July, 2019 https://www.extremeviruses.org/stedman-lab-lassen-2019 daily 0.75 2019-09-12 https://images.squarespace-cdn.com/content/v1/5a4af594e9bfdfa8208274d5/1568326136749-GR2MPGIVO8QCSP6AH2KQ/IMG_5653.JPG Stedman Lab, Lassen 2019 Stedman Lab Group, Boiling Springs Lake, Lassen Volcanic National Park, 3 August 2019 https://www.extremeviruses.org/omsi-after-dark daily 0.75 2019-09-12 https://images.squarespace-cdn.com/content/v1/5a4af594e9bfdfa8208274d5/1568326996863-SNHWW767DBP6YKQ15KO6/KAV_1_31Jul19+%281%29.jpg OMSI After Dark Ken doing outreach at OMSI After Dark https://www.extremeviruses.org/nichole-presents-at-pacnow-2019 daily 0.75 2019-08-23 https://images.squarespace-cdn.com/content/v1/5a4af594e9bfdfa8208274d5/1566533496849-MOCMG7K4DZS0NMX0TDM2/Nicole_PacNow19.JPG Nichole Presents at PacNow 2019 Nichole presenting her poster. https://www.extremeviruses.org/stedman-lab-talks daily 0.75 2019-03-22 https://images.squarespace-cdn.com/content/v1/5a4af594e9bfdfa8208274d5/1553298272156-UGLAW6XNO7K4LULTMHL8/Diana_LLL.JPG Stedman Lab Talks https://images.squarespace-cdn.com/content/v1/5a4af594e9bfdfa8208274d5/1553298283922-O8JDK9ERT0OVO8BHRP4M/Nichole_LLL.JPG Stedman Lab Talks https://images.squarespace-cdn.com/content/v1/5a4af594e9bfdfa8208274d5/1553298296602-PPGDSRUOWV8N4QIRSN9E/Nacho_VirusesFromHeavenandHell.JPG Stedman Lab Talks https://www.extremeviruses.org/stedman-lab-video daily 0.75 2019-09-12 https://www.extremeviruses.org/ssv1-grant-update daily 0.75 2019-06-23 https://images.squarespace-cdn.com/content/v1/5a4af594e9bfdfa8208274d5/1561227920286-VCB8G3GGWQIIGMZG1EQK/IMG_5302.JPG SSV1 Grant Update Stanley Celebrating at Group Meeting. https://www.extremeviruses.org/ase-poster-2019 daily 0.75 2019-09-12 https://images.squarespace-cdn.com/content/v1/5a4af594e9bfdfa8208274d5/1568326460108-3WPNTP62BMUO75614UND/SummerSSV_CrewASE.JPG ASE Poster 2019 Poster Session at Apprenticeships in Science and Engineering Symposium University of Portland, 16 Aug, 2019 https://www.extremeviruses.org/ase-writeup daily 0.75 2019-11-12 https://images.squarespace-cdn.com/content/v1/5a4af594e9bfdfa8208274d5/1573529597772-MPKBMBVKPVMJHWR7EP64/CorannaASE.png ASE Writeup https://www.extremeviruses.org/sigma-xi-art-and-science daily 0.75 2019-11-29 https://www.extremeviruses.org/new-page-5 daily 0.75 2020-04-01 https://images.squarespace-cdn.com/content/v1/5a4af594e9bfdfa8208274d5/1585763757088-JJSIII3MS9E8LQ013P20/Rita_TEM.jpg Rita Clare gets fellowship Rita at the TEM https://www.extremeviruses.org/new-page-4 daily 0.75 2020-04-01 https://images.squarespace-cdn.com/content/v1/5a4af594e9bfdfa8208274d5/1585763255647-PIAK4PQFOQUUBQ3DEPFY/EllisHeadShot.png Ellis Torrance gets Fellowship https://www.extremeviruses.org/equity-statement daily 0.75 2020-11-09 https://www.extremeviruses.org/george-passes-his-dissertation-defense daily 0.75 2021-06-06 https://images.squarespace-cdn.com/content/v1/5a4af594e9bfdfa8208274d5/1623002613896-C4WYZ7CEHNRHE86XXF2T/Screen+Shot+2021-06-04+at+5.38.08+PM.png George Passes his Dissertation Defense! George Passes his Dissertation Defense, 4 June 2021 https://www.extremeviruses.org/ken-omsi-science-pub daily 0.75 2022-07-11 https://images.squarespace-cdn.com/content/v1/5a4af594e9bfdfa8208274d5/c4e17cc4-df3b-42c5-aba4-5defafd3b6e4/OMSI_Sci_Pub.png Ken OMSI Science Pub https://www.extremeviruses.org/home-alt-bedford daily 0.75 2025-05-12 https://images.squarespace-cdn.com/content/v1/52a74d9ae4b0253945d2aee9/1390500612440-PGQSIY8KL9F7T5VIXQLO/chambers-2.jpg Home Alt A better world Starts with a choice Contact Us https://images.squarespace-cdn.com/content/v1/52a74d9ae4b0253945d2aee9/1390500503574-5BAPXZO9U06UV61P49UO/chambers1.jpg Home Alt Sustainability Starts with you Join Us https://images.squarespace-cdn.com/content/v1/52a74d9ae4b0253945d2aee9/1390500933384-LII4SRIPHFYOUFX0UCXC/chambers-4.jpg Home Alt A better world Starts with a choice Join Us https://images.squarespace-cdn.com/content/v1/52a74d9ae4b0253945d2aee9/1390504287124-HQ8GNX1JCE22V047MSQ9/chambers-8.jpg Home Alt Preserve Nature Join Us